This research was commissioned by the Accelerating Localisation Through Partnerships programme – a multi-agency consortium programme funded by the European Commission’s Civil Protection and Humanitarian Aid department (ECHO) over two years (2017-2019) – to establish what operational elements of partnerships between local, national and international NGOs are most likely to foster localisation of humanitarian action. The research was underpinned by a mixed methods approach using qualitative and quantitative data collection approaches. In-depth consultations were conducted in three locations across South Sudan to reach a varied sample of local and national actors: Wau, Bor, and Juba City. In total, 96 NGOs were consulted for this research in South Sudan; 85% of which were local or national NGOs. The findings reflect experiences from a rich diversity of local and national NGOs in South Sudan and provide valuable insights that can assist humanitarian organisations in ensuring partnership practices accelerate localisation of humanitarian action. Findings are also relevant for those funding humanitarian response, in particular signatories of the Grand Bargain.